Cryosections were fixed in 4% paraformaldehyde for 1 hour and stained with Masson’s trichrome (Sigma Aldrich), as described previously4 (link). Images were taken with a 20x digital slide scanner (0.5×0.5μm2 resolution, Aperio ScanScope XT, Leica).
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Masson's Trichrome Staining of Cryosections
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Cryosections
Paraformaldehyde
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Corresponding Organization :
Other organizations : Columbia University, University of Manchester, Lung Institute, The Ohio State University Wexner Medical Center
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Variable analysis
independent variables
- Cryosections were fixed in 4% paraformaldehyde for 1 hour
- Images were taken with a 20x digital slide scanner (0.5×0.5μm2 resolution, Aperio ScanScope XT, Leica)
- Staining with Masson's trichrome (Sigma Aldrich), as described previously
- The use of a positive control (stained sample) and a negative control (unstained sample) is not explicitly mentioned.
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