In a subset of participants (n=8 HC, n=8 MDD), two 3 mm cutaneous vascular tissue samples were obtained from the ventral forearm under local anesthesia (2% lidocaine without epinephrine) via punch biopsy, immediately snap frozen in liquid nitrogen, and stored at −80°C until analysis34 (link), 40 (link). Protein abundance/expression was determined by Western blot and total reactive oxygen species and reactive nitrogen species (ROS+RNS) production was quantified fluorometrically (Cell Biolabs Inc, San Diego, CA, USA). The relative contribution of superoxide to total ROS+RNS was assessed by pretreatment with the SOD mimetic MnTBAP (Sigma-Aldrich). Details are provided in the Online Data Supplement .
Protocol detail
Cutaneous Vascular Tissue Biopsy and Analysis
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Biopsy
Cell
Cutaneous tissue
Epinephrine
Forearm
Frozen
Lidocaine
Local anesthesia
Mntbap
Nitrogen
Protein
Reactive nitrogen species
Reactive oxygen species
Superoxide
Supplement
Vascular
Western blot
Corresponding Organization :
Other organizations : Penn State Milton S. Hershey Medical Center, Johns Hopkins Medicine, Johns Hopkins University, Pennsylvania State University
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Variable analysis
independent variables
- Diagnostic group (HC vs. MDD)
- Protein abundance/expression (determined by Western blot)
- Total reactive oxygen species and reactive nitrogen species (ROS+RNS) production (quantified fluorometrically)
- Relative contribution of superoxide to total ROS+RNS (assessed by pretreatment with the SOD mimetic MnTBAP)
- Local anesthesia (2% lidocaine without epinephrine) used for tissue sampling
- Tissue samples obtained from the ventral forearm via punch biopsy
- Tissue samples immediately snap frozen in liquid nitrogen and stored at −80°C until analysis
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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