Antibiotic Resistance Profiling of S. aureus

The phenotypic pattern of antibiotic resistance of S. aureus bacteria was investigated using the disk diffusion method on Mueller–Hinton agar (Merck, Germany).13 (link) Principles of the Clinical Laboratory Standard Institute (CLSI) were used for this purpose.14 Diverse kinds of antibiotic agents including aminoglycosides (amikacin (30 µg/disk) and gentamicin (10 µg/disk)), fluoroquinolones (levofloxacin (5 µg/disk) and ciprofloxacin (5 µg/disk)), lincosamides (clindamycin (2 µg/disk)), macrolides (erythromycin (15 µg/disk) and azithromycin (15 µg/disk)), penicillins (penicillin (10 µg/disk)), tetracyclines (doxycycline (30 µg/disk) and tetracycline (30 µg/disk)), phenicols (chloramphenicol (30 µg/disk)), folate pathway inhibitors (trimethoprim–sulfamethoxazole (25 µg/disk)), and ansamycins (rifampin (5 µg/disk)) were used for this goal (Oxoid, UK). The method was completed using the protocol characterized previously.14
S. aureus (ATCC 43300 and ATCC 29213) was used as the quality control organism in antimicrobial susceptibility determination.

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Torki Baghbaderani Z., Shakerian A, & Rahimi E. (2020). Phenotypic and Genotypic Assessment of Antibiotic Resistance of Staphylococcus aureus Bacteria Isolated from Retail Meat. Infection and Drug Resistance, 13, 1339-1349.

Publication 2020

Mueller–Hinton agar amikacin levofloxacin clindamycin erythromycin azithromycin penicillin doxycycline chloramphenicol trimethoprim sulfamethoxazole rifampin

Agar Amikacin Aminoglycosides Ansamycins Antibiotic agents Antimicrobial Azithromycin Bacteria antibiotic resistance Chloramphenicol Ciprofloxacin Clindamycin Clinical laboratory Diffusion Doxycycline Erythromycin Fluoroquinolones Folate inhibitors Gentamicin Levofloxacin Lincosamides Macrolides Penicillin Phenotypic Rifampin Susceptibility Tetracycline Tetracyclines Trimethoprim sulfamethoxazole

Corresponding Organization : Islamic Azad University of Shahrekord

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Variable analysis

independent variables

Antibiotic agents including aminoglycosides (amikacin and gentamicin), fluoroquinolones (levofloxacin and ciprofloxacin), lincosamides (clindamycin), macrolides (erythromycin and azithromycin), penicillins (penicillin), tetracyclines (doxycycline and tetracycline), phenicols (chloramphenicol), folate pathway inhibitors (trimethoprim–sulfamethoxazole), and ansamycins (rifampin)

dependent variables

Phenotypic pattern of antibiotic resistance of S. aureus bacteria

control variables

S. aureus (ATCC 43300 and ATCC 29213) used as the quality control organism in antimicrobial susceptibility determination
Disk diffusion method on Mueller–Hinton agar (Merck, Germany)
Principles of the Clinical Laboratory Standard Institute (CLSI) used for the experiment

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