Whey Hydrolysate Modulates NF-κB Cytokine Production
Corresponding Organization : FrieslandCampina (Netherlands)
Variable analysis
- NF-κB inhibitor celastrol (10 μM)
- Intact whey protein (2 mg/ml)
- Whey protein hydrolysates (2 mg/ml)
- TNFα levels
- IL-10 levels
- THP-1 monocytes differentiated into THP-1 macrophages
- RPMI medium supplemented with 10% decomplemented FBS, 2mM L-glutamine, 1mM sodium pyruvate, 0.05mM 2-mercaptoethanol, 60 μg/ml gentamycin sulfate, and 2.2 μg/ml amphotericin B
- PMA (100 ng/ml) used to differentiate cells into macrophages
- Cell seeding density (1x10^6 cells/well)
- Incubation time (24 hours)
- Positive control: Cells incubated with medium (no NF-κB inhibitor)
- Negative control: Not explicitly mentioned
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