Transient expression assay for BrpHMA2::LUC in tobacco

BrpHMA2 fused to the firefly LUC gene (BrpHMA2::LUC) was constructed in pGreenII 0800-LUC as a reporter, and BrpNACs or other AREBs were constructed in the pGreenII 62-SK vector as effectors. pGreenII 62-SK and pGreenII 0800-LUC vectors were used for transient expression assays in tobacco leaves. The reporter and effector were transiently expressed in tobacco leaves mediated by Agrobacterium GV3101 (pSoup). Agrobacterium-infected plants were cultivated under low irradiance for 24 hours and then transferred to light for an additional 2 days [45 (link)]. The LUC signal in the transfected leaves was detected with a CCD camera (Vilber Newton 7.0).

Free full text: Click here

Liu S., Li L., Deng Y., Bai Y., Sun C., Huang S., Zhou J., Shi L., Yang X., Li L., Chen X, & Tang Y. (2022). BrpNAC895 and BrpABI449 coregulate the transcription of the afflux-type cadmium transporter BrpHMA2 in Brassica parachinensis. Horticulture Research, 9, uhac044.

Publication 2022

Newton 7.0

Agrobacterium Assays Firefly Gene Light Plants Tobacco Transient Vectors

Corresponding Organization : Shenzhen University

Other organizations : Jilin Academy of Traditional Chinese Medicine, South China Normal University, Zhaoqing University, Nanjing Agricultural University, University of California, Riverside

Top 5 similar protocols

Variable analysis

independent variables

BrpHMA2::LUC reporter
BrpNACs or other AREBs as effectors

dependent variables

LUC signal in the transfected leaves

control variables

PGreenII 62-SK vector
PGreenII 0800-LUC vector
Agrobacterium GV3101 (pSoup)
Cultivation under low irradiance for 24 hours followed by 2 days of light

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!