Ultrastructural Characterization of Fusarium Wilt

Foc TR4 was grown on the PDA medium with 4 × EC₅₀ of extract at 28°C for 5 days. The collected mycelia were fixed with 2.5% (v/v) of glutaraldehyde overnight at 4°C. DMSO (10%, v/v) treatment was used as a control. Agar plugs (5 mm in diameter) with Foc TR4 were cut from the edge of a 3-day-old fungal medium. The mycelial sections were prepared according to Jing et al. (2020) (link). Morphological characteristics of Foc TR4 mycelia were detected using SEM. The effect of strain 8ZJF-21 extract on the cellular ultrastructure of Foc TR4 was observed by a transmission electron microscope (TEM, JEM-1400 Flash, Hitachi Limited, Tokyo, Japan) according to Wei et al. (2020) (link). Four replicates were used per treatment and each experiment was repeated three times.