Immunofluorescence Labeling Protocol

Immunofluorescence labeling was conducted according to our standard protocols (Meng et al., 2020 (link)). At the indicated time post-AE-treatment, cells were fixed with 4% PFA for 10 min in PBS and permeabilized for 15 min with 0.2% Triton X-100 in PBS. After blockade of nonspecific binding by incubation of cells for 30 min with 2% bovine serum albumin (BSA) in PBS, samples were incubated with the appropriate primary antibodies and subsequently incubated with fluorescein-labeled secondary antibodies (ThermoFisher). Confocal fluorescence images were acquired at the confocal microscope (Olympus). All image data shown are representative of randomly selected fields from at least three replicates.