We conducted RNA extraction using TRIzol reagent (Invitrogen) as previously described [33 (link)]. We obtained cDNA using a pTRUEscript 1st Strand cDNA Synthesis Kit (Aidlab, Beijing, China). We conducted RT-qPCR with the 2× SYBR Green qPCR Mix (Aidlab) on the ABI 7900HT sequence detection machine (Thermo Fisher Scientific, Waltham, MA, USA). Expression fold differences were determined via the 2−ΔΔCT method.
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