Citronellol Extraction from Citrus hystrix Leaves

Citrus hystrix DC leaves powder obtained from Khaolaor Company (Samut Prakan, Thailand) was macerated in three different solvents sequentially, as previously described (Buakaew et al., 2021a (link),b (link)). Crude hexane, crude ethyl acetate, and crude ethanol extracts were obtained. citronellol, the active compound was identified from C. hystrix leaves extract (Buakaew et al., 2021a (link),b (link)). For in vitro experiments, citronellol was purchased from Sigma-Aldrich Company (St. Louis, MO, United States). They were stored at 4°C for future experiments.

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Buakaew W., Pankla Sranujit R., Noysang C., Krobthong S., Yingchutrakul Y., Thongsri Y., Potup P., Daowtak K, & Usuwanthim K. (2022). Proteomic Analysis Reveals Proteins Involved in the Mode of Action of β-Citronellol Identified From Citrus hystrix DC. Leaf Against Candida albicans. Frontiers in Microbiology, 13, 894637.

Publication 2022

citronellol

Citronellol Citrus hystrix Crude extracts Ethanol Ethyl acetate Hexane Hystrix Powder Solvents

Corresponding Organization : Naresuan University

Other organizations : Rajamangala University of Technology, Mahidol University, Kasetsart University, National Science and Technology Development Agency

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Variable analysis

independent variables

Solvents (hexane, ethyl acetate, ethanol) used for sequential maceration of Citrus hystrix DC leaves powder

dependent variables

Crude extracts obtained (hexane, ethyl acetate, ethanol)

control variables

Citrus hystrix DC leaves powder obtained from Khaolaor Company (Samut Prakan, Thailand)
Maceration procedure as previously described (Buakaew et al., 2021a, b)

positive controls

Citronellol, the active compound identified from C. hystrix leaves extract (Buakaew et al., 2021a, b)

negative controls

None specified

Annotations

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