DSS-Induced Colitis in Female C57BL/6N Mice

Fifty-six female C57BL/6N mice (CLEA Japan, Inc., Tokyo, Japan) aged 11–12 weeks were used in our study. Male mice were excluded in the current study due to their high mortality rates from DSS-induced colitis [31 (link)]. The mice were housed in a pathogen-free environment at a temperature of approximately 23 ± 3 °C, relative humidity of 55 ± 10%, and a 12 h light/dark cycle. The mice had free access to their diet and drinking water throughout the experiment. The ethics approval document of this animal experiment was provided on 6 February 2018.

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Agista A.Z., Rusbana T.B., Islam J., Ohsaki Y., Sultana H., Hirakawa R., Watanabe K., Nochi T., A.r.d.i.a.n.s.y.a.h., Budijanto S., Yang S.C., Koseki T., Aso H., Komai M, & Shirakawa H. (2021). Fermented Rice Bran Supplementation Prevents the Development of Intestinal Fibrosis Due to DSS-Induced Inflammation in Mice. Nutrients, 13(6), 1869.

Publication 2021

C57BL/6N mice

C57bl mice Colitis Diet Female Humidity Male Mice Pathogen

Corresponding Organization : Tohoku University

Other organizations : Sultan Ageng Tirtayasa University, Bakrie University, IPB University, Taipei Medical University, Yamagata University

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Variable analysis

independent variables

DSS-induced colitis

dependent variables

Mortality rates

control variables

Female C57BL/6N mice
Age of mice (11-12 weeks)
Housing conditions (pathogen-free environment, temperature, humidity, light/dark cycle)
Access to diet and drinking water

controls

Male mice were excluded due to their high mortality rates from DSS-induced colitis

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