Fluorescent Antibody Labeling for Organelle Imaging

Antibodies for immunofluorescence were mouse IgG2b anti-human CD63 (clone TS63b^{35 (link)} 1/100, available upon request to E. Rubinstein: eric.rubinstein@inserm.fr) and mouse IgG1 anti-human CD9 (clone TS9^{36 (link)} 1/100) (commercially available at Diaclone or Abcam), mouse IgG1 anti-human EEA1 (BD Transduction Laboratories, clone 14/EEA1, 1/1000), mouse IgG2a anti-human RAB5 (BD Transduction Laboratories, clone 1/Rab5, 1/100), rabbit anti-human RAB7 (Cell Signaling, D95F2, 1/100), mouse IgG1 anti-human LAMP1 (Developmental Studies Hybridoma Bank, H4A3, 1/400), goat anti-mouse IgG2b Alexafluor 647 (Invitrogen, 1/300), goat anti-mouse IgG1 Alexafluor 488 (Invitrogen 1/300), goat anti-mouse IgG Alexafluor 568 (Invitrogen, 1/200), goat anti-rabbit IgG Alexafluor 568 (Invitrogen, 1/200), goat anti-rabbit IgG Alexafluor 647 (Invitrogen, 1/200).

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Mathieu M., Névo N., Jouve M., Valenzuela J.I., Maurin M., Verweij F.J., Palmulli R., Lankar D., Dingli F., Loew D., Rubinstein E., Boncompain G., Perez F, & Théry C. (2021). Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. Nature Communications, 12, 4389.

Publication 2021

goat anti-mouse IgG2b Alexafluor 647 goat anti-mouse IgG1 Alexafluor 488 goat anti-mouse IgG Alexafluor 568 goat anti-rabbit IgG Alexafluor 568 goat anti-rabbit IgG Alexafluor 647

Alexafluor 647 Anti igg Antibodies Clone Goat Human Hybridoma Igg1 Igg2a Igg2b Immunofluorescence Lamp1 Mouse Rabbit

Corresponding Organization :

Other organizations : Université Paris Sciences et Lettres, Inserm, Institut Curie, Centre National de la Recherche Scientifique, Institut de Psychiatrie et Neurosciences de Paris, Université Paris Cité, Centre d'Immunologie et des Maladies Infectieuses, Sorbonne Université

Top 5 similar protocols

Variable analysis

independent variables

Mouse IgG2b anti-human CD63 (clone TS63b) (1/100)
Mouse IgG1 anti-human CD9 (clone TS9) (1/100)
Mouse IgG1 anti-human EEA1 (1/1000)
Mouse IgG2a anti-human RAB5 (1/100)
Rabbit anti-human RAB7 (1/100)
Mouse IgG1 anti-human LAMP1 (1/400)
Goat anti-mouse IgG2b Alexafluor 647 (1/300)
Goat anti-mouse IgG1 Alexafluor 488 (1/300)
Goat anti-mouse IgG Alexafluor 568 (1/200)
Goat anti-rabbit IgG Alexafluor 568 (1/200)
Goat anti-rabbit IgG Alexafluor 647 (1/200)

dependent variables

Localization of CD63, CD9, EEA1, RAB5, RAB7, and LAMP1 proteins

control variables

Concentration of antibodies used (dilutions provided)

Annotations

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