Postmortem Transbronchial Cryobiopsy for Lung Sampling

Postmortem transbronchial cryobiopsy samples were obtained as previously described [11 (link)]. After patient death, patient lung aeration was maintained by applying a constant positive airway pressure equal to the positive end-expiratory pressure received before death. As per national regulations, death was ascertained with a continuous 20-min flat electrocardiogram, while the sampling procedure was initiated within 30 min from death. Cryobiopsies were obtained with a 1.7 mm cryoprobe (ErbeCryo^®, Erbe Elektromedizin GmbH, Tuebingen, Germany) inserted through the operative channel of a single use flexible video-bronchoscope (Ambu^® aScope^TM, Ambu, Ballerup, Denmark) and operated for 10–11 s. Biopsies were collected and immediately fixed in 10% formalin for each lobe: right lower lobe (RLL), right middle lobe (RML), right upper lobe (RUL), left lower lobe (LLL) and left upper lobe (LUL).

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Ball L., Barisione E., Mastracci L., Campora M., Costa D., Robba C., Battaglini D., Micali M., Costantino F., Cittadini G., Patroniti N., Pelosi P., Fiocca R, & Grillo F. (2021). Extension of Collagen Deposition in COVID-19 Post Mortem Lung Samples and Computed Tomography Analysis Findings. International Journal of Molecular Sciences, 22(14), 7498.

Publication 2021

ErbeCryo Ambu® aScopeTM

Biopsies Bronchoscope Electrocardiogram Formalin Lung Patient Positive end expiratory pressure Postmortem Pressure

Corresponding Organization : Ospedale Policlinico San Martino

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Variable analysis

independent variables

Duration of cryobiopsy procedure (10-11 s)

dependent variables

Lung tissue sample obtained from different lobes (RLL, RML, RUL, LLL, LUL)

control variables

Positive airway pressure maintained at the level of positive end-expiratory pressure received before death
Time between death confirmation (20-min flat electrocardiogram) and sampling procedure initiation (within 30 min)
Cryoprobe size (1.7 mm)
Use of single-use flexible video-bronchoscope (Ambu® aScope™)

controls

Positive control: Maintaining lung aeration by applying constant positive airway pressure
Negative control: Not specified

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