Bony specimens were fixed with 10% freshly prepared formalin for 24 h then decalcified using 10% formic acid for 7 days. Embedding was done using paraffin wax; bone blocks were sectioned by microtome for serial sections of 4 μm. Sections were placed on slides and stained using hematoxylin and eosin (H&E) stain, and Trichrome stain. The morphological characters of the newly formed bone were evaluated.
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