DNA extraction was conducted using the DNAeasy Qiagen kit, while the DNA was quantified using the GloMax Plate Reader System (Promega) using the QuantiFluor® dsDNA System (Promega) chemistry. Samples were submitted for metagenomic analysis to a third party (CosmoID) which has validated methods and software17 (link),18 (link) for library preparation, sequencing and cloud-based computing for data analysis19 .
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Metagenomic Analysis of Microbial DNA
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Corresponding Organization :
Other organizations : Case Western Reserve University, Cleveland Clinic, The Ohio State University
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Variable analysis
independent variables
- DNA extraction method: DNAeasy Qiagen kit
- DNA quantification: GloMax Plate Reader System (Promega) using the QuantiFluor® dsDNA System (Promega) chemistry
- Metagenomic analysis: Library preparation, sequencing, and cloud-based computing for data analysis
- Not explicitly mentioned
- Positive control: Not specified
- Negative control: Not specified
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