Cytokine Profiling of Infected Murine Bladder

Mice were infected as described above and were euthanised 24-h post infection. The bladders were collected and homogenised in the presence of a protease inhibitor cocktail (Roche). The samples were spun at 12,000 x g for 20 min at 4 °C, and supernatants were stored at −80 °C. Cytokine measurements were performed on 4–9 samples per group using the Bio-Plex Pro Mouse Cytokine 23–Plex Group 1 kit as per manufacturer’s instructions (Bio-Rad).

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Nhu N.T., Rahman M.A., Goh K.G., Kim S.J., Phan M.D., Peters K.M., Alvarez-Fraga L., Hancock S.J., Ravi C., Kidd T.J., Sullivan M.J., Irvine K.M., Beatson S.A., Sweet M.J., Irwin A.D., Vukovic J., Ulett G.C., Hasnain S.Z, & Schembri M.A. (2024). A convergent evolutionary pathway attenuating cellulose production drives enhanced virulence of some bacteria. Nature Communications, 15, 1441.

Publication 2024

protease inhibitor cocktail

Corresponding Organization :

Other organizations : University of Queensland, Griffith University

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Variable analysis

independent variables

Mice were infected as described above

dependent variables

Cytokine measurements were performed on 4–9 samples per group

control variables

Mice were euthanised 24-h post infection
The bladders were collected and homogenised in the presence of a protease inhibitor cocktail (Roche)
The samples were spun at 12,000 x g for 20 min at 4 °C
Supernatants were stored at −80 °C

controls

No positive or negative controls were explicitly mentioned in the protocol.

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