NanoLuc concentrations in cell culture supernatants were quantified with the Nano-Glo Luciferase Assay System (N1110, Promega). 10 μL of cell culture supernatant was mixed with 10 μL of buffer/substrate mix (50:1) in 384-well plates (781076, Greiner Bio-One), which were briefly centrifuged at 1200 rpm and incubated for 5 min at room temperature. Luminescence intensity was measured using a Tecan Spark multiplate reader (Tecan AG).
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