Reliable 1,5-AG Measurement in Stored Samples

1,5-AG (GlycoMark, Winston-Salem, NC) was measured using a Roche Modular P800 system in 2012–2013 in stored serum specimens obtained at ARIC visit 2. The interassay CV was 5%. The reliability coefficient for N = 610 masked duplicate specimen pairs was 0.99. Previous studies have shown this 1,5-AG assay to be highly reliable even in long-term stored samples (8 (link),36 (link)).

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Selvin E., Rawlings A., Lutsey P., Maruthur N., Pankow J.S., Steffes M, & Coresh J. (2015). Association of 1,5-Anhydroglucitol With Cardiovascular Disease and Mortality. Diabetes, 65(1), 201-208.

Publication 2015

Modular P800 system

Assay Serum

Corresponding Organization : Johns Hopkins Medicine

Other organizations : University of Minnesota

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

1,5-AG (GlycoMark, Winston-Salem, NC) measured using a Roche Modular P800 system

control variables

Interassay CV was 5%
Reliability coefficient for N = 610 masked duplicate specimen pairs was 0.99
Previous studies have shown this 1,5-AG assay to be highly reliable even in long-term stored samples (8 and 36 months)

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