Protocol detail

Biochemical Identification of Suspected Salmonella

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Suspected Salmonella colonies from nutrient agar (Oxoid, England CM0003-500G) were picked up and its biochemical characteristics were determined using triple sugar iron agar (Oxoid, England CM277-500G), lysine iron agar (Oxoid, England CM0381-500G), Simmon’s citrate agar (Himedia, India CM0129-500G), urea slant (Himedia, India M111A-500G), and sulfide indole motility agar (Oxoid, England S12-500G).3 (link)

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Alemu A., Regassa F., Kebede N., Ambachew R., Girma M., Asefa Z, & Tsegaye W. (2022). Magnitude and Antimicrobial Susceptibility Profile of Salmonella Recovered from Export Abattoirs Located in East Shewa, Ethiopia. Infection and Drug Resistance, 15, 1353-1365.

Publication 2022

nutrient agar triple sugar iron agar lysine iron agar Simmon’s citrate agar sulfide indole motility agar

Agar Citrate Indole Iron Lysine Motility Nutrient Salmonella Sugar Sulfide Urea

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Variable analysis

independent variables

Suspected Salmonella colonies from nutrient agar (Oxoid, England CM0003-500G)

dependent variables

Biochemical characteristics of the suspected Salmonella colonies

control variables

Triple sugar iron agar (Oxoid, England CM277-500G)
Lysine iron agar (Oxoid, England CM0381-500G)
Simmon's citrate agar (Himedia, India CM0129-500G)
Urea slant (Himedia, India M111A-500G)
Sulfide indole motility agar (Oxoid, England S12-500G)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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