Cellular Uptake of Hematoporphyrin

Cellular uptake study of HpD was performed as follow; Cells were incubated for overnight on 12-well plate at 2 × 10⁵ cells/well. The medium was exchanged to flesh one which contained 20 µM of HpD and incubated for 0.5, 1, 3 and 6 h. After incubation, cells were dissolved with 100 µl of self-prepared RIPA buffer.^{(13 (link))} The cell homogenates were transferred to 96-well plate and then the fluorescence intensity of HpD was measured by a Varioskan micro plate reader (Thermo Fisher Scientific K.K., Kanagawa, Japan). The measurement wavelength of excitation and emission were 415 nm and 625 nm, respectively.