Confocal microscopy was used to observe the dynamic surface binding and internalization of mAb and ADC in NET cells following our established protocol.(38 (link), 39 ) Specifically, BacMam GFP Transduction Control was used to stain the cytoplasm and nucleus, and the AF647-mAb or AF647-ADC was used to target cells. The stained cells were observed using an Olympus 1X-81 confocal microscope with a laser scanning head (Olympus IX81, Center Valley, PA). The MitoSox images were recorded and analyzed offline via ImageJ software.
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Visualizing mAb and ADC Binding and Internalization in NET Cells
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Other organizations : University of Alabama at Birmingham
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Variable analysis
independent variables
- MAb (monoclonal antibody)
- ADC (antibody-drug conjugate)
- Dynamic surface binding
- Internalization of mAb and ADC in NET cells
- BacMam GFP Transduction Control to stain the cytoplasm and nucleus
- AF647-mAb or AF647-ADC to target cells
- Olympus 1X-81 confocal microscope with a laser scanning head
- Positive control: BacMam GFP Transduction Control to stain the cytoplasm and nucleus
- Negative control: Not explicitly mentioned
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