Kidney Ultrastructure Analysis by TEM

The cortices of the mouse kidney were fixed in 2.5% v/v glutaraldehyde solution for 24 h, then processed for TEM examination according to routine TEM processing and staining protocols for tissues in the City of Hope Electron Microscopy and Atomic Force Microscopy Core^{13 (link)}. Ultra-thin sections were cut using a Leica Ultra-cut UCT ultramicrotome. TEM was performed on FEI Tecnai 12 TEM equipped with a Gatan Ultrascan 2 K CCD camera. Average GBM thickness was quantified in ~100 measurements and area of 40 mitochondrion was measured in each group using Image-Pro Premier 9.2 software.

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Kato M., Abdollahi M., Tunduguru R., Tsark W., Chen Z., Wu X., Wang J., Chen Z.B., Lin F.M., Lanting L., Wang M., Huss J., Fueger P.T., Chan D, & Natarajan R. (2021). miR-379 deletion ameliorates features of diabetic kidney disease by enhancing adaptive mitophagy via FIS1. Communications Biology, 4, 30.

Publication 2021

Ultra-cut UCT ultramicrotome Tecnai 12 TEM Ultrascan 2 K CCD camera

Atomic force microscopy Cortices of kidney Electron microscopy Glutaraldehyde Mitochondrion Mouse Staining tissues Thin sections Ultramicrotome

Corresponding Organization : City of Hope

Other organizations : California Institute of Technology

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Variable analysis

independent variables

Glutaraldehyde concentration (2.5% v/v)

dependent variables

Average GBM thickness
Area of 40 mitochondrion

control variables

Duration of fixation (24 h)
TEM processing and staining protocols
Tissue source (mouse kidney cortices)

controls

Positive control: Not specified
Negative control: Not specified

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