Chondrogenic Differentiation of hBMSCs

3D pellets were formed using 2.5×10⁵ human bone marrow-derived mesenchymal stem cells (hBMSCs). Chondrogenesis was initiated in serum-free chondrogenic differentiation medium. From day 14 onwards, cell pellets were maintained either in the standard chondrogenic differentiation medium or in the presence of T₃ (10 nM) or IOP (10 μM). For additional details, see online supplemental methods.

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Bomer N., den Hollander W., Ramos Y.F., Bos S.D., van der Breggen R., Lakenberg N., Pepers B.A., van Eeden A.E., Darvishan A., Tobi E.W., Duijnisveld B.J., van den Akker E.B., Heijmans B.T., van Roon-Mom W.M., Verbeek F.J., van Osch G.J., Nelissen R.G., Slagboom P.E, & Meulenbelt I. (2014). Underlying molecular mechanisms of DIO2 susceptibility in symptomatic osteoarthritis. Annals of the Rheumatic Diseases, 74(8), 1571-1579.

Publication 2014

Bone marrow mesenchymal stem cells Cell Chondrogenic Human Pellets Serum

Corresponding Organization : Leiden University Medical Center

Other organizations : Netherlands Consortium for Healthy Ageing, Delft University of Technology, Erasmus University Rotterdam, Erasmus MC

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Variable analysis

independent variables

Presence of T3 (10 nM)
Presence of IOP (10 μM)

dependent variables

Chondrogenic differentiation of hBMSCs

control variables

Standard chondrogenic differentiation medium

controls

Positive control: Chondrogenic differentiation medium
Negative control: Not mentioned

Annotations

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