We re-dissolved dry protein-free lipid-free extracts in 600 μL of 20 mM deuterated phosphate buffer (pH 7.2) containing 2 × 10−5 M sodium 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS) as an internal standard. The 1H NMR spectra were obtained using an AVANCE III HD 700 MHz NMR spectrometer (Bruker BioSpin, Ettlingen, Germany) equipped with a 16.44 Tesla Ascend cryomagnet at the Center of Collective Use “Mass spectrometric investigations” SB RAS as described in [26 (link)].
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