Cardiac Hypertrophy Modeling and Analysis

All experiments involving animals were approved by the Animal Care and Use Committee of Renmin Hospital, Wuhan University, China. An expanded Methods section is available in the online-only Data Supplement, which includes Reagents, Mice Used in This Study,^{17 (link)} Aortic Banding,^{1 (link),8 (link),9 (link),15 (link)} Echocardiography and Hemodynamic Measurements,^{1 (link),8 (link),9 (link)} Histological Analysis, Cultured Neonatal Rat Cardiac Myocytes and Recombinant Adenoviral Vectors,^{1 (link)} Measurement of the Protein/DNA Ratio, Atrial Natriuretic Peptide (ANP) Quantification Assays, Immunofluorescence Analysis, Quantitative Real-Time PCR and Western Blotting,^{1 (link),8 (link),9 (link)} IKKβ Activity Assays, Luciferase Reporter Assays,^{23 (link),24 (link)} Hydroxyproline Assay,^{25 (link)} Immunoprecipitation, Glutathione S-transferase (GST) Pull-down Assay, Confocal Microscopy, and Statistical Analysis.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Jiang D.S., Liu Y., Zhou H., Zhang Y., Zhang X.D., Zhang X.F., Chen K., Gao L., Peng J., Gong H., Chen Y., Yang Q., Liu P.P., Fan G.C., Zou Y, & Li H. (2014). Interferon Regulatory Factor 7 Functions as a Novel Negative Regulator of Pathological Cardiac Hypertrophy. Hypertension (Dallas, Tex. : 1979), 63(4), 713-722.

Publication 2014

Adenoviral Animal Aortic Assay Atrial natriuretic peptide Cardiac myocytes Confocal microscopy Echocardiography Glutathione s transferase Hemodynamic Hydroxyproline Ikkβ Immunofluorescence Immunoprecipitation Luciferase Mice Neonatal Protein dna Pull Quantitative real time pcr Supplement Vectors

Corresponding Organization :

Other organizations : Huazhong University of Science and Technology, Zhongshan Hospital, Union Hospital, University of Minnesota, University of Cincinnati Medical Center, University of Alabama at Birmingham, Fudan University, University of Ottawa, Wuhan University

Top 5 similar protocols

Protocol cited in 11 other protocols

Variable analysis

independent variables

Aortic banding

dependent variables

Cardiac function (measured by echocardiography and hemodynamic measurements)
Histological changes
Protein/DNA ratio
Atrial natriuretic peptide (ANP) levels
Gene and protein expression (measured by qRT-PCR and Western blotting)
IKKβ activity
Luciferase reporter activity
Hydroxyproline levels

control variables

Mice used in the study

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!