Electroacupuncture Stimulation Protocol

The EA group received EA stimulation on the 15th day of the experiment (Figure 1B). Mice were anesthetized by inhalation of isoflurane (1.5%–3.0%) via an anesthesia machine. EA was performed with a disperse-dense wave mode for 20 min once per day for 7 days, with electrical current range of 1–2 mA, and a frequency of 2/15 Hz by using a SDZ-IV electronic instrument (Huatuo Brand). EA stimulation was performed at the abdominal RN12 (Zhongwan) and dorsal BL21 (Weishu) acupoints by inserting a 0.18 × 13 mm acupuncture needle. The RN12 acupoint is located on the anterior median line of the upper abdomen, 10 mm below the xiphisternal synchondroses. The BL21 acupoint is located under the spinous process of the 12th thoracic vertebra.