Modulation of Regulatory T Cells in APPPS1 Mice

Modulation of Tregs was carried out as previously described [21 (link)]. Briefly, for Treg depletion, 4- to 5-week-old APPPS1 mice and wild-type littermates were injected intraperitoneally with 200 µg of either purified (BioXCell) or ascite-derived anti-CD25 depleting monoclonal antibody (clone PC61) diluted in phosphate-buffered saline (PBS). Control mice were injected with either PBS or 200 µg of purified control IgG1 (BioXCell). Similar treatments were repeated 4 weeks after the first injection. For amplification of Tregs, 6-week-old APPPS1 mice and wild-type littermates were treated daily for 10 consecutive days with intraperitoneal injections of either PBS or 50,000 IU of recombinant human IL-2 (Proleukin; Novartis) diluted in PBS. Additional daily treatments for 5 days were repeated every 3 weeks. The efficiency of treatments for Treg depletion or amplification was monitored by flow cytometry as previously described [21 (link)].