CaCo-2 Cell Glycomics and Proteomics

In this study, the O-glycome and proteome of CaCo-2 cell line were analyzed from three biological replicates. The samples were collected and analyzed from five different time points, starting from day 5, to days 7, 14, 21, and 24, postconfluence in two groups: spontaneous differentiation and butyrate-stimulated differentiation. Differences between groups and time points were tested using two-way ANOVA with significance level of α = 0.05 both for glycomics and proteomics datasets. Data analysis and visualization was performed using in-house developed “R’’ scripts. To enable use of principal component analysis, imputation of minimum positive number (0.0001) was performed.