Immunostaining of Cardiac Tissue Markers

The primary antibodies in this study were as follows: goat anti-ephrin-B1 (R&D Systems; AF473); mouse anti-α sarcomeric actinin (Sigma-Aldrich, Cat# A7732, RRID:AB_2221571 or Abcam, Cat# ab68167, RRID:AB_11157538); mouse anti-connexin 43 (Millipore; MAB3067); rabbit anti-N-cadherin (Epitomics; 2019-1); rabbit anti-desmoplakin 1/2 (ARP American Research Products, Cat# 03-61003, RRID:AB_1541118); rabbit anti-claudin-5 (Acris Antibodies, Cat# DP157, RRID:AB_978124); mouse anti-GAPDH (Abcam, Cat# ab9484, RRID:AB_307274), mouse anti-Ryanodine Receptor (Abcam, Cat# ab2868, RRID:AB_2183051), and mouse anti-caveolin-3 (BD Biosciences, Cat# 610420, RRID:AB_397800). The secondary fluorescent antibodies used in this study were as follows: donkey anti-goat Alexa Fluor488 (Molecular Probes, Cat# A-11055, RRID:AB_2534102); donkey anti-mouse Alexa Fluor488 (Cat# A-21202, RRID:AB_141607), goat anti-rabbit Oregon-Green488 (Cat# 011038), donkey anti-goat Alexa Fluor568 (Cat# A-11057, RRID:AB_2534104), and donkey anti-rat Alexa Fluor568 (Cat# A-11007, RRID:AB_10561522), all obtained from Thermo Fisher Scientific. Cell nuclei were stained with 4′,6-Diamidino-2-phenylindole dihydrochloride-DAPI (Sigma-Aldrich, Cat# 32670) or TO-PRO–3 Iodide (642/661) (Invitrogen; Cat# T3605).

Free full text: Click here

Karsenty C., Guilbeau-Frugier C., Genet G., Seguelas M.H., Alzieu P., Cazorla O., Montagner A., Blum Y., Dubroca C., Maupoint J., Tramunt B., Cauquil M., Sulpice T., Richard S., Arcucci S., Flores-Flores R., Pataluch N., Montoriol R., Sicard P., Deney A., Couffinhal T., Senard J.M, & Galés C. (2023). Ephrin-B1 regulates the adult diastolic function through a late postnatal maturation of cardiomyocyte surface crests. eLife, 12, e80904.

Publication 2023

goat anti-ephrin-B1 mouse anti-α sarcomeric actinin mouse anti-connexin 43 mouse anti-GAPDH donkey anti-goat Alexa Fluor488 donkey anti-mouse Alexa Fluor488 donkey anti-goat Alexa Fluor568 4′,6-Diamidino-2-phenylindole dihydrochloride-DAPI TO-PRO–3 Iodide (642/661)

Actinin Anti b1 Antibodies Cadherin Caveolin 3 Cell nuclei Claudin 5 Connexin 43 Dapi Desmoplakin 2 Donkey Ephrin Fluorescent antibodies Gapdh Goat Iodide Molecular probes Mouse Rabbit Ryanodine receptor Sarcomeric To pro 3

Corresponding Organization :

Other organizations : Centre Hospitalier Universitaire de Toulouse, Institut des Maladies Métaboliques et Cardiovasculaires, Inserm, Université de Toulouse, University of Virginia, Université de Bordeaux, Université de Montpellier, Centre National de la Recherche Scientifique, Institut de génétique et de développement de Rennes, Université de Rennes, Centre Hospitalier Universitaire de Bordeaux

Top 5 similar protocols

Variable analysis

independent variables

Primary antibodies used in the study

dependent variables

Not explicitly mentioned

control variables

Antibody types and sources used for immunostaining
Cell nuclei staining dyes used

controls

Positive control: None specified
Negative control: None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!