Gastric Cancer Tissue Sample Collection

All the clinical samples were collected from the First Affiliated Hospital of Nanchang University. In the current study, fresh GC tumor tissues and adjacent normal gastric tissues were collected from patients who underwent surgical resection, blood samples were collected from GC patients diagnosed with primary GC without previously treatment, and paraffin-embedded GC tissues and adjacent noncancerous gastric tissues were obtained from patients who underwent resection between January 2014 and December 2015. Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh blood using Ficoll-Hypaque density gradient centrifugation (Biochrom, Berlin, Germany) according to a previous study [16 (link)]. All of the included patients provided informed consent and signed an agreement. Approval for this study was obtained from the Ethics Committee of the First Affiliated Hospital of Nanchang University.

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Fang W., Shi C., Wang Y., Song J, & Zhang L. (2021). microRNA-128-3p inhibits CD4+ regulatory T cells enrichment by targeting interleukin 16 in gastric cancer. Bioengineered, 13(1), 1025-1038.

Publication 2021

Ficoll-Hypaque density gradient centrifugation

Blood Density gradient centrifugation Ficoll Gastric Hospital ethics committee Human Hypaque Paraffin Patients Peripheral blood mononuclear cells Surgical Tissues Tumor

Corresponding Organization : Nanchang University

Other organizations : Third Affiliated Hospital of Nanchang University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Samples collected from patients who underwent surgical resection
Blood samples collected from GC patients diagnosed with primary GC without previously treatment
Paraffin-embedded GC tissues and adjacent noncancerous gastric tissues obtained from patients who underwent resection between January 2014 and December 2015

controls

Positive control: Adjacent normal gastric tissues collected from the same patients as the GC tumor tissues
Negative control: Not explicitly mentioned

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