The detection of Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), Campylobacter rectus, Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia (previously T. forsythensis), and Treponema denticola in pooled plaque samples was evaluated by real-time qPCR, as described,20 (link),21 (link) using primers specific for the hypervariable segments of the 16S rRNA genes of each bacterium (Table 1 ). The percentage of the total flora for each species was calculated by dividing the number of target organisms by the total number of bacteria as determined by qPCR using 16S rRNA primers that reacted with all bacterial species. Data were represented using a patient-based assessment.
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Quantifying Oral Pathogenic Bacteria
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16s rrna
Actinobacillus actinomycetemcomitans
Bacterial
Campylobacter rectus
Fusobacterium nucleatum
Patient
Plaque
Porphyromonas gingivalis
Prevotella intermedia
Primers
Rrna genes
Tannerella forsythia
Treponema denticola
Corresponding Organization : University of Michigan–Ann Arbor
Other organizations : University of California, Berkeley, Material Sciences (United States), Sandia National Laboratories California
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Variable analysis
independent variables
- Detection of Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), Campylobacter rectus, Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia (previously T. forsythensis), and Treponema denticola in pooled plaque samples
- Percentage of the total flora for each species, calculated by dividing the number of target organisms by the total number of bacteria as determined by qPCR using 16S rRNA primers that reacted with all bacterial species
- Primers specific for the hypervariable segments of the 16S rRNA genes of each bacterium (Table 1)
- Real-time qPCR method as described in references 20 and 21
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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