Generation and Validation of Transgenic Mice

Female WT C57BL/6 mice were purchased from Jackson Laboratory (Catalog #000664, Bar Harbor, Maine). The generation of H-2K^b LoxP parental mice and the MHC class I deficient CMV-Cre parental mice are detailed in our previous work(24 (link)). Genotypes were confirmed by PCR for Cre-Recombinase using primers from the Jackson Laboratory. Flow cytometry for surface H-2K^b MHC class I surface protein ensured the appropriate identity of all experimental animals. Mice were bred and maintained in the animal facility at Mayo Clinic under IACUC guidelines. Animal handling and all procedures were done in the manner approved by the Mayo Clinic Institutional Animal Care and Use Committee.