RNA was extracted from EndoC-βH1 cells, and sequencing was performed using Illumina RiboMethSeq Protocol (ebook ISBN 978-1-4939-6807-7, Chapter 12) (46 (link)). The library was prepared by the NEB-Next multiplex small RNA library prep set for Illumina. The libraries were loaded and sequenced on Illumina NextSeq500 sequencer in the sequencing core facility at Lund University Diabetes Centre. Transcript reads were mapped to the human transcriptome (Gencode Release 30) and quantified with Salmon (v.0.14.0). DEGs were identified with DESeq2 (v.1.25.10). Detailed schematic of RNA sequencing protocol and analysis is depicted in (Fig. S4 ).
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