Antibody Characterization for CTD Modifications

Antibodies α-tubulin (T6557) (Sigma), HA (3F10) (Roche), H3K4me3 (Ab8580) (Abcam) were commercially available. Monoclonal antibodies against CTD Tyr1P (3D12),²⁹ (link) Ser2P (3E10), Ser5P (3E8), Ser7P (4E12) and Rpb1 (Pol 3.3),³⁰ (link) Pes1 (8E9), Bop1 (6H11)³¹ (link) were described previously. Pol 3.3 recognizes an epitope in Rpb1 outside of the CTD. The rat monoclonal antibody 1F5 (IgG2a) against CTD K7me1/2 was produced toward the CTD-peptide (YSPTSPKme2YpSPTSPSC) coupled to ovalbumin for immunization (Peptide Specialty Laboratories GmbH, Heidelberg, Germany).

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Voss K., Forné I., Descostes N., Hintermair C., Schüller R., Maqbool M.A., Heidemann M., Flatley A., Imhof A., Gut M., Gut I., Kremmer E., Andrau J.C, & Eick D. (2015). Site-specific methylation and acetylation of lysine residues in the C-terminal domain (CTD) of RNA polymerase II. Transcription, 6(5), 91-101.

Publication 2015

HA (3F10) Ab8580

Antibodies Epitope H3k4me3 Igg2a Immunization Monoclonal antibody Ovalbumin Peptide α tubulin

Corresponding Organization : Center for Integrated Protein Science Munich

Other organizations : Ludwig-Maximilians-Universität München, Urologische Klinik München, Centre d’Immunologie de Marseille-Luminy, Aix-Marseille Université, Institut de Génétique Moléculaire de Montpellier, Centre National de la Recherche Scientifique, Centro Nacional de Análisis Genómico

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Variable analysis

independent variables

Antibodies α-tubulin (T6557) (Sigma)
HA (3F10) (Roche)
H3K4me3 (Ab8580) (Abcam)
Monoclonal antibodies against CTD Tyr1P (3D12)
Ser2P (3E10)
Ser5P (3E8)
Ser7P (4E12)
Rpb1 (Pol 3.3)
Pes1 (8E9)
Bop1 (6H11)
Rat monoclonal antibody 1F5 (IgG2a) against CTD K7me1/2

dependent variables

Not explicitly mentioned

control variables

Pol 3.3 recognizes an epitope in Rpb1 outside of the CTD
1F5 (IgG2a) was produced toward the CTD-peptide (YSPTSPKme2YpSPTSPSC) coupled to ovalbumin for immunization

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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