Carbon-11 based Pittsburgh Compound-B for positron emission tomography (PiB-PET) based estimation of individual brain Aβ load (Mathis et al., 2003 (link); Klunk et al., 2004 (link); Solbach et al., 2005 (link)) was performed as reported earlier by our group at the PET Center of the Division of Nuclear Medicine, Zürich University Hospital utilizing a GE PET/CT Discovery scanner (Steininger et al., 2014 (link)). In brief, an individual dose of 350 MBq of (11)carbon-labeled PiB was injected into the cubital vein. Images were corrected for attenuation using a low-dose CT. Standard quantitative filtered back projection algorithm including necessary corrections was applied.
Cerebral amyloid deposition values were extracted using a standard routine as implemented in PMOD Brain Tool software-package (PNEURO, Version 3.4, PMOD Technologies Ltd, Zürich, Switzerland). Late frame (minutes 50–70) values were standardized by the cerebellar gray matter value, resulting in 3D-volumes of PiB-PET retention (matrix dimensions: 128 × 128 × 47, voxel size: 2.34 × 2.34 × 3.27 mm).
Cerebral amyloid deposition values were extracted using a standard routine as implemented in PMOD Brain Tool software-package (PNEURO, Version 3.4, PMOD Technologies Ltd, Zürich, Switzerland). Late frame (minutes 50–70) values were standardized by the cerebellar gray matter value, resulting in 3D-volumes of PiB-PET retention (matrix dimensions: 128 × 128 × 47, voxel size: 2.34 × 2.34 × 3.27 mm).
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