Blood samples were collected from the antecubital vein of all participants in the morning under fasting conditions. They were stored in vacuum tubes containing EDTA (ethylene diamine tetraacetic acid) and coagulation tubes. A range of haematological and biochemistry tests (Table 2 ) were conducted on fresh samples at the central laboratory of the Staff Hospital of Jidong oil-field of Chinese National Petroleum. Fasting blood glucose was measured with the hexokinase/glucose-6-phosphate dehydrogenase method. Cholesterol and triglyceride concentrations were determined by enzymatic methods (Mind Bioengineering Co. Ltd, Shanghai, China). Blood samples were also measured using an auto-analyzer (Hitachi 747; Hitachi, Tokyo, Japan) at the central laboratory of the Staff Hospital of Jidong oil-field of Chinese National Petroleum. For all participants, serum creatinine, cholesterol, high-density lipoproteins (HDL-C), low-density lipoproteins (LDL-C), triglycerides and glucose levels were assessed. In subgroup analysis studies, various biomarkers of blood cells, serum and plasma were measured: C-reactive protein, homocysteine, estrogens, androgens, vitamin D, lipoprotein-associated phospholipase A2 (Lp-PLA2), insulin, and glycosylated hemoglobin HbA1c.
Haematology, biochemistry and biological specimen banking in the COACS
| Analysate | |
|---|---|
| Red blood cells | Haemoglobin |
| Red corpuscle count | |
| Haematocrit | |
| Mean corpuscular volume | |
| Mean corpuscular | |
| Haemoglobin concentration | |
| Red blood cell distribution width | |
| White blood cells | White cell count Total count |
| Differential count | |
| Platelets | Platelets Count |
| Mean platelet volume | |
| Urea | Urine specific gravity |
| Ery | |
| Urea nitrogen | |
| Uric acid (UA) | |
| Creatinine (Cr) | |
| Urine protein | |
| Liver function tests (plasma) | Alkaline phosphatise |
| Alanine transaminase (ALT) | |
| Aspartate aminotransferase (AST) | |
| Phosphatise Transglutaminase (TG) | |
| Liver function tests (serum) | HBsAg |
| Anti-HBs | |
| HBeAg | |
| Anti-HBe | |
| Anti-HBc | |
| Lipids (plasma) | Total cholesterol (TC) |
| Total bilirubin (TBIL) | |
| Triglycerides (TG) | |
| Low density lipoprotein (LDL) | |
| Very Low density lipoprotein (VLDL) | |
| General chemistry (plasma) | C-reactive protein |
| Homocysteine | |
| Steroids | |
| Glucose | |
| Insulin | |
| Glycosylated hemoglobin | |
| Bio-specimen banking | |
| White blood cells | DNA, RNA extraction and analyses |
| Serum | Pedtidome profiling |
| Plasma | Glycome |
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