ELISA-Based Complement Inhibition Analysis

In vitro analysis of C2scFv and C2scFv-Crry binding was determined by ELISA using cardiolipin (Sigma Aldrich, MO) as described for C2 IgM nAb.^{10 (link)} In vitro complement inhibitory activity of C2scFv/C2scFv-Crry was determined as described.^{8 (link),12 (link)}

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Li C., Patel K., Tu Z., Yang X., Kulik L., Alawieh A., Allen P., Cheng Q., Wallace C., Kilkenny J., Kwon J., Gibney B., Cantu E., Sharma A., Pipkin M., Machuca T., Emtiazjoo A., Goddard M., Holers V.M., Nadig S., Christie J., Tomlinson S, & Atkinson C. (2021). A novel injury site-natural antibody targeted complement inhibitor protects against lung transplant injury. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 21(6), 2067-2078.

Publication 2021

cardiolipin

Cardiolipin Elisa Inhibitory

Corresponding Organization : Ralph H. Johnson VA Medical Center

Other organizations : Tongji Hospital, Huazhong University of Science and Technology, University of Colorado Denver, University of Pennsylvania, Papworth Hospital

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Variable analysis

independent variables

C2scFv
C2scFv-Crry

dependent variables

Binding to cardiolipin (measured by ELISA)
Complement inhibitory activity

control variables

Experimental methods (ELISA and complement inhibition assays) as described in the referenced publications

positive controls

C2 IgM nAb (as per reference 10)

negative controls

Not explicitly mentioned

Annotations

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