Liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (LC/Q-TOF) analysis was performed on an Agilent 1260 LC system [consisting of a G1322A degasser, a G1312B binary pump, a G1367E thermostated autosampler, G1316A thermostated column compartment, and a G4212B diode array detector (DAD)] (Agilent Technologies, Santa Clara, CA, USA) coupled to an ultra high definition quadrupole time-of-flight mass spectrometer Model 6540 (Agilent Technologies, Santa Clara, CA, USA) equipped with a dual source electrospray ionization ion source. The sample treatment, LC separation and Q-TOF parameters were conducted according to previously described methods (Tang et al., 2015 (link)).
The analytes were separated on an Agilent C18 reverse-phase column (100 × 1.8 mm, particle size of 3.5 μm). The binary mobile phase composed of 2% methanol and 98% water (containing 0.2% ammonium acetate and 0.1% acetic acid) was set at a constant flow rate of 300 μL/min and the column temperature was kept at 30°C, and the sample volume was 2 μL. Q-TOF parameters were as previously reported (Tang et al., 2015 (link)).
The analytes were separated on an Agilent C18 reverse-phase column (100 × 1.8 mm, particle size of 3.5 μm). The binary mobile phase composed of 2% methanol and 98% water (containing 0.2% ammonium acetate and 0.1% acetic acid) was set at a constant flow rate of 300 μL/min and the column temperature was kept at 30°C, and the sample volume was 2 μL. Q-TOF parameters were as previously reported (Tang et al., 2015 (link)).
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