Urinary and Plasma Electrolyte Analysis

Urinary and plasma K⁺ concentrations were measured using Jenway PFP7 Flame photometer (Bibby Scientific). Plasma was separated by centrifugation at 1300 g in Vacutainer Plus SST plastic tubes with clot activator and gel for plasma separation (BD; Cat. # 367988). Urinary pH was measured using MI‐410 pH microelectrode (Microelectrodes Inc.). Urinary creatinine concentration was assessed with QuantiChrom Creatinine Assay Kit (BioAssay Systems; Cat. # DICT‐500) utilizing improved Jaffe method (Mamenko et al., 2012 (link)). Aldosterone was measured using an enzymatic immunoassay kit (Cayman Chemical; Cat. # 501090) in accordance with the vendor's protocol. Kidney injury marker 1 (KIM1) was measured using a commercially available kit (R&D System; Cat. # RKM100).