Characterization of NB Cell Lines

Two NB cell lines, namely SH-SY5Y (ATCC CRL-2266) and SK-N-BE(2) (ATCC CRL-2271), were selected for the xenograft studies. SH-SY5Y is a TP53 wild type, non-MYCN-amplified cell line with no chromosome 1p loss of heterozygosity. The SK-N-BE(2) cell line is amplified for MYCN, carries a TP53 mutation (p.C135F), and is heterozygous for loss of chromosome 1p [70 (link)]. Expression of MYCN protein in these cell lines was reported earlier [42 (link)]. NB cells were cultured under standard conditions, as described previously [41 (link)].

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Aminzadeh-Gohari S., Feichtinger R.G., Vidali S., Locker F., Rutherford T., O’Donnel M., Stöger-Kleiber A., Mayr J.A., Sperl W, & Kofler B. (2017). A ketogenic diet supplemented with medium-chain triglycerides enhances the anti-tumor and anti-angiogenic efficacy of chemotherapy on neuroblastoma xenografts in a CD1-nu mouse model. Oncotarget, 8(39), 64728-64744.

Publication 2017

SH-SY5Y SK-N-BE(2)

Cell lines Cells Chromosome Loss of heterozygous Mutation Mycn protein Tp53 Xenograft

Corresponding Organization : Paracelsus Medical University

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Variable analysis

independent variables

Cell lines: SH-SY5Y and SK-N-BE(2)

dependent variables

Not explicitly mentioned

control variables

Culture conditions for NB cells as described previously [41]

controls

No positive or negative controls were explicitly mentioned in the provided information.

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