HPLC Analysis of Polyphenolic Compounds

The polyphenolic composition in the DE was analyzed via high-performance liquid chromatography (Waters Scientific Ltd., Mississauga, ON, Canada) using a YMC-Triart C18 column (250 × 4.6 mm, 5 μm, YMC, Kyoto, Japan). The mobile phase was water and acetonitrile containing 0.2% formic acid, the flow rate was 0.8 mL/min, and the injection volume was 10 μL. The measurement wavelength was 260 nm for gallic acid, 3,4-dihydroxybenzoic acid, and rutin; 310 nm for chlorogenic acid, p-coumaric acid, caffeic acid, trans-ferulic acid, and apigenin; and 365 nm for quercetin and kaempferol [21 (link)].

Free full text: Click here

Ko K., Ahn Y., Cheon G.Y., Suh H.J., Cho Y.J., Park S.S, & Hong K.B. (2023). Effects of Dendropanax morbiferus Leaf Extract on Sleep Parameters in Invertebrate and Vertebrate Models. Antioxidants, 12(10), 1890.

Publication 2023

Triart C18 column

Acetonitrile Apigenin Caffeic acid Chlorogenic acid Dihydroxybenzoic acid Formic acid Gallic acid High performance liquid chromatography Kaempferol P coumaric acid Quercetin Rutin Trans ferulic acid

Corresponding Organization : Jeju National University

Other organizations : Korea Food Research Institute, Korea University, Jeju TechnoPark

Top 5 similar protocols

Variable analysis

independent variables

Mobile phase composition (water and acetonitrile containing 0.2% formic acid)
Flow rate (0.8 mL/min)
Injection volume (10 μL)

dependent variables

Polyphenolic composition in the DE

control variables

Column (YMC-Triart C18 column, 250 × 4.6 mm, 5 μm)
Measurement wavelengths (260 nm, 310 nm, 365 nm)

controls

No positive or negative controls were explicitly mentioned in the protocol.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!