Biomarker Assessment in Fasting Blood

Venous blood samples were collected after 8 h overnight fasting. Serum ferritin was measured with an immunoradiometric assay using a 1470 WIZARD^® automatic gamma counter (PerkinElmer, Waltham, MA, USA) in the central laboratory. Fasting plasma concentrations of glucose and lipid were measured enzymatically using a Hitachi Automatic Analyzer 7600 (Hitachi, Tokyo, Japan). Glycated hemoglobin levels were determined by high-performance liquid chromatography with an automated HLC-723G7 analyzer (Tosoh Corporation, Tokyo, Japan). Serum creatinine levels were measured colorimetrically (Hitachi Automatic Analyzer 7600) and eGFR was calculated using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation [17 (link)]. To obtain the UACR, urinary albumin was measured in spot urine using the immunoturbidimetric method and urinary creatinine was measured using the colorimetric method.

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Oh I.H., Choi E.Y., Park J.S, & Lee C.H. (2016). Association of Serum Ferritin and Kidney Function with Age-Related Macular Degeneration in the General Population. PLoS ONE, 11(4), e0153624.

Publication 2016

1470 WIZARD Hitachi Automatic Analyzer 7600 HLC-723G7 analyzer

Albumin Colorimetric Creatinine Egfr Ferritin Gamma Glucose Glycated hemoglobin High performance liquid chromatography Immunoradiometric assay Immunoturbidimetric Lipid Plasma Serum Urinary Venous blood

Corresponding Organization : Hanyang University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum ferritin
Fasting plasma concentrations of glucose and lipid
Glycated hemoglobin levels
Serum creatinine levels
Estimated glomerular filtration rate (eGFR)
Urinary albumin-to-creatinine ratio (UACR)

control variables

8 h overnight fasting period before blood sample collection

controls

None explicitly mentioned

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