Investigating Lysophosphatidic Acid Receptor Knockout Mice

C57BL/6 (Stock No: 000664, Jackson Laboratories), Lpar2^−/− mice (41 (link)) (gift from Dr. Jerold Chun, Scripps Research Institute), Lpar5^−/− mice (39 (link)), CD45.1 OT-I mice (gift from Ross Kedl, Ph.D., University of Colorado), Lpar5^−/− OT-I mice, and Rag2^−/− mice (Stock No: 008449, Jackson Laboratories), Enpp2^+/− mice (42 (link)) (gift from Dr. Susan Smyth, University of Kentucky) were bred and housed at the University of Colorado Anschutz Medical Campus Vivarium (Aurora, CO, USA). OT-I mice (43 (link)) harbor CD8 T cells with a transgenic Vβ5Vα2 TCR specific for the SIINFEKL peptide from chicken ovalbumin (residues 257–264) but also recognize SIIGFEKL with lower affinity (44 (link)). Splenocytes from Lpar6^−/− and Nur77^GFP OT-I mice (45 (link)) mice were gifted from Dr. Iain McKillop (Atrium Health, Carolinas Medical Center University) and Dr. Ross Kedl, respectively. All procedures with animals were approved by the University of Colorado Institutional Animal Care and Use Committee.