In Vivo CD4+ and CD8+ T Cell Depletion

BALB/c mice (n = 8–10) immunized with pE1D2 or pcTPANS1 were depleted from CD4⁺ or CD8⁺ cells upon inoculation of in-house produced ascitic fluids containing anti-CD4 (clone GK 1.5) or anti-CD8 antibodies (clone 56-3.7) (39 (link)). Animals were inoculated by the intraperitoneal route (i.p.) with 20 μL of ascitic fluids on days 4 and 2 before DENV2 challenge. T cell depletion was monitored by flow cytometry in blood cells stained with anti-CD3 FITC (clone 145-2C11), anti-CD8 PerCP (clone 33-6.7) and anti-CD4 PE (clone RM4-5) antibodies (BD Biosciences), and evaluated on FlowJo software.

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Pinto P.B., Assis M.L., Vallochi A.L., Pacheco A.R., Lima L.M., Quaresma K.R., Pereira B.A., Costa S.M, & Alves A.M. (2019). T Cell Responses Induced by DNA Vaccines Based on the DENV2 E and NS1 Proteins in Mice: Importance in Protection and Immunodominant Epitope Identification. Frontiers in Immunology, 10, 1522.

Publication 2019

anti-CD3 FITC (clone 145-2C11), anti-CD4 PE (clone RM4-5)

Animals Anti antibodies Anti cd3 Antibodies Ascitic fluids Balb c mice Blood cells Cd8 cells Clone Fitc Flow cytometry Inoculation T cell

Corresponding Organization :

Other organizations : Fundação Oswaldo Cruz

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Variable analysis

independent variables

Depletion of CD4+ or CD8+ cells upon inoculation of in-house produced ascitic fluids containing anti-CD4 (clone GK 1.5) or anti-CD8 antibodies (clone 56-3.7)

dependent variables

T cell depletion monitored by flow cytometry in blood cells stained with anti-CD3 FITC (clone 145-2C11), anti-CD8 PerCP (clone 33-6.7) and anti-CD4 PE (clone RM4-5) antibodies

control variables

BALB/c mice (n = 8–10) immunized with pE1D2 or pcTPANS1

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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