Biochemical Data Acquisition Protocol

Biochemical data acquiring Protocols was also described in detail in our previous publication [15 (link)]. Appointments were scheduled for blood collection. Fasting venous blood draws were performed at The People’s Hospital of Zhengzhou. All blood samples were sent to the central laboratory of The People’s Hospital of Zhengzhou. The blood samples were either disposed of within 3 h or stored at 4 °C for as long as 2 days. FPG (fasting plasma glucose, FPG) testing was performed via an electrochemical luminescence immunoassay. Serum total cholesterol (TC), HDLC, TG, and low-density lipoprotein cholesterol (LDLC) were each measured using an autoanalyzer (Toshiba, Japan). Serum creatinine (Scr) was measured using overnight fasting venous blood samples, via Jaffe’s kinetic method. Uric acid was detected by uricase-based spectrophotometry.