Pulmonary Deposition of 5FU Powders

The in vitro profile of pulmonary deposition of the powders was determined using the Dry Powder Inhaler Andersen Cascade Impactor (ACI-DPI, Apparatus D European Pharmacopoeia, Copley Scientific Limited, Erweka, Germany), consisting of 8 stages (0–7) with rotary impactor plates connected to a flow controller and vacuum pump. Samples of 5FU-MS (30 mg), 5FU-NS (20 mg), and the drug 5FU pure (21 mg) were transferred to hard gelatin capsules (size 3), which were introduced into the single-dose inhaler (Aerolizer^®, Novartis, Basel, Switzerland) and perforated twice. This experiment was conducted under controlled inhalation rate of 28.3 L·min⁻¹ for 4 s and a pressure of 4 kPa. After inhalation, the particles retained in each stage were rinsed off with ultrapure water. The concentration of 5FU was quantified by HPLC-UV according to the extraction method described in Section 4.3.2. The Fine Particle Dose (FPD), Fine Particle Fraction (FPF), Respirable Fraction (RF), and Dose Issued (DI) of inhaled powders for pulmonary absorption were determined according to the equations described by Meenach et al. (2013) [46 (link)].

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Zatta K.C., Frank L.A., Reolon L.A., Amaral-Machado L., Egito E.S., Gremião M.P., Pohlmann A.R, & Guterres S.S. (2018). An Inhalable Powder Formulation Based on Micro- and Nanoparticles Containing 5-Fluorouracil for the Treatment of Metastatic Melanoma. Nanomaterials, 8(2), 75.

Publication 2018

Aerolizer

Capsules Drug Dry powder inhaler European Gelatin Hplc Inhalation rate Inhaler Powders Pressure Pulmonary Pulmonary absorption Vacuum

Corresponding Organization : Universidade Federal do Rio Grande do Sul

Other organizations : Universidade Federal do Rio Grande do Norte, Universidade Estadual Paulista (Unesp)

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Variable analysis

independent variables

Type of powder (5FU-MS, 5FU-NS, 5FU pure)

dependent variables

Fine Particle Dose (FPD)
Fine Particle Fraction (FPF)
Respirable Fraction (RF)
Dose Issued (DI)

control variables

Inhalation rate (28.3 L·min^-1)
Inhalation duration (4 s)
Inhalation pressure (4 kPa)
Hard gelatin capsule size (size 3)
Single-dose inhaler (Aerolizer®, Novartis, Basel, Switzerland)

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