Characterization of MDA-MB-231 Breast Cancer Cells

Cell lines used for characterization were grown aseptically to 30–60% confluence at 37 °C in a humidified atmosphere of 5% CO₂. MDA-MB-231 (triple-negative breast carcinoma, ATCC® HTB-26™) cells were grown in DMEM medium supplemented with 10% inactivated FBS and 1% penicillin/streptomycin. Adherent cells were dissociated with TrypLE express cell dissociation reagent (Gibco) and resuspended in complete media. For experiments related to Fig. 2, the cell line used was MDA-MB-231 cells that stably expressed firefly luciferase-enhanced green fluorescent protein (FLuc-eGFP), a generous gift from the Paulmurugan lab at Stanford University [69 (link)].