The visualization of growing PTs with aniline blue staining utilizes the ability of this fluorochrome to bind to the callose contained in the pollen grain wall and PTs.
The pollinated pistils fixed in acetic alcohol (90% ethanol and acetic acid, 3 : 1) were used in the experiments. Pistils were macerated in 20% KOH alcohol solution for 20–40 min, washed twice with distilled water, and stained with 0.01% aniline blue solution for 30–40 min. The stained pistils were placed into a drop of glycerin mixed with water (1 : 1) on a glass slide, covered with a cover glass, gently squashed, and examined using a Zeiss Axioplan (Carl Zeiss, Germany) fluorescence microscope with 365 nm excitation filter and 420 nm emission filter. At least 200 PTs were examined in each variant of the experiment.
The pollinated pistils fixed in acetic alcohol (90% ethanol and acetic acid, 3 : 1) were used in the experiments. Pistils were macerated in 20% KOH alcohol solution for 20–40 min, washed twice with distilled water, and stained with 0.01% aniline blue solution for 30–40 min. The stained pistils were placed into a drop of glycerin mixed with water (1 : 1) on a glass slide, covered with a cover glass, gently squashed, and examined using a Zeiss Axioplan (Carl Zeiss, Germany) fluorescence microscope with 365 nm excitation filter and 420 nm emission filter. At least 200 PTs were examined in each variant of the experiment.
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