Comprehensive Immunohistochemical Profiling

Immunohistochemistry was performed as described earlier in a two-step staining method by a Techmate 500 immunostainer (DAKO).10 (link) Antibodies used from DAKO were as follows: cytokeratin proteins (clone AE1-AE3), vimentin (V9), desmin (D33), smooth muscle actin (1A4), muscle-specific actin (HHF35), S-100 (#GA504), melanosome (HMB45), CD31 (JC0A), CD34 (QBEnd), CD68 (KP1), CD3 (#GA503), CD20 (L26), CD45LCA (2B11+PD7/26), estrogen receptor (EP1), progesterone receptor (PgR636), factor VIII (#GA527), Ki67 (MIB-1), kappa chain (#GA506), gamma chain (#GA512), calretinin (DAK-Calret 1), WT1 (6F-H2), epithelial membrane antigen (E29), Myogenin (clone F5D), smooth muscle actin (1A4) and E-cadherin (NCH38).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Salvador-Coloma C., Saigí M., Díaz-Beveridge R., Penín R.M., Pané-Foix M., Mayordomo E., Melián M., Schuler M., García Del Muro X, & Font de Mora J. (2019). Identification Of Actionable Genetic Targets In Primary Cardiac Sarcomas. OncoTargets and therapy, 12, 9265-9275.

Publication 2019

Techmate 500

Actin Antibodies Calretinin Clone Cytokeratin Desmin E cadherin Epithelial membrane antigen Estrogen receptor Factor viii Gamma Immunohistochemistry Melanosome Mib 1 Muscle Myogenin Progesterone receptor Proteins S 100 Smooth muscle Staining method Vimentin

Corresponding Organization : Instituto de Investigación Sanitaria La Fe

Other organizations : Institut Català d'Oncologia, Institut d'Investigació Biomédica de Bellvitge, Bellvitge University Hospital

Top 5 similar protocols

Variable analysis

independent variables

Immunohistochemistry method: two-step staining method by a Techmate 500 immunostainer (DAKO)

dependent variables

Expression of cytokeratin proteins, vimentin, desmin, smooth muscle actin, muscle-specific actin, S-100, melanosome, CD31, CD34, CD68, CD3, CD20, CD45LCA, estrogen receptor, progesterone receptor, factor VIII, Ki67, kappa chain, gamma chain, calretinin, WT1, epithelial membrane antigen, Myogenin, smooth muscle actin, and E-cadherin

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!