Generation of MITRG and MISTRG Mice

The knockin replacement (from ATG to STOP codon) of individual loci (encoding TPO, IL-3/GM-CSF or M-CSF) in the Rag2^-/-Il2rg^-/- 129xBalb/c (N2) genetic background was performed using Velocigene Technology in collaboration with Regeneron Pharmaceuticals, as reported previously^{16 (link)-18 (link)}. Human SIRPα expression was achieved by BAC-transgenesis in the same genetic background^{28 (link)}. We intercrossed these strains by conventional breeding to obtain MITRG (M-CSF^h/h IL-3/GM-CSF^h/h TPO^h/hRag2^-/-Il2rg^-/-) and MISTRG (M-CSF^h/h IL-3/GM-CSF^h/h hSIRPA^tg TPO^h/hRag2^-/-Il2rg^-/-) mice. We maintained them under specific pathogen free conditions with continuous treatment with enrofloxacin in the drinking water (Baytril, 0.27 mg/ml). MITRG and MISTRG are viable for 1 year or more and have normal fertility at least until 8 months old. NOD Scid Il2rg^-/-(NSG) mice were obtained from The Jackson Laboratory.
MITRG and MISTRG mice have been deposited at The Jackson Laboratory. [AU: these stock numbers do not retrieve any mice on the JAX website—if the numbers will not be functional by the time this paper is published please delete them from this manuscript] [AR: we don't have any information on when these strains will appear on the JAX website. The transfer of the mice is currently in progress]

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Rongvaux A., Willinger T., Martinek J., Strowig T., Gearty S.V., Teichmann L.L., Saito Y., Marches F., Halene S., Palucka A.K., Manz M.G, & Flavell R.A. (2014). Development and function of human innate immune cells in a humanized mouse model. Nature biotechnology, 32(4), 364-372.

Publication 2014

NOD Scid Il2rg-/-(NSG) mice

Baytril Codon Enrofloxacin Fertility Genetic Genetic background Gm csf Human Il2rg M csf Mice Pharmaceuticals Rag2 Scid Sirpα Specific pathogen free Strains

Corresponding Organization : Howard Hughes Medical Institute

Other organizations : University Hospital of Zurich, Yale Cancer Center

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Variable analysis

independent variables

Knockin replacement (from ATG to STOP codon) of individual loci (encoding TPO, IL-3/GM-CSF or M-CSF)
Human SIRPα expression achieved by BAC-transgenesis

dependent variables

Viability of MITRG and MISTRG mice for 1 year or more
Normal fertility of MITRG and MISTRG mice at least until 8 months old

control variables

Genetic background: Rag2-/- Il2rg-/- 129xBalb/c (N2)
Maintenance under specific pathogen free conditions
Continuous treatment with enrofloxacin in the drinking water

positive controls

NOD Scid Il2rg-/-(NSG) mice obtained from The Jackson Laboratory

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