Evaluating Colorectal Cancer Organoid Responses

Patient-derived organoids were obtained from resected tumours of patients with colorectal cancer. All patients provided informed consent before tumour collection, and the study was approved by the Human Research Ethics Committee (HREC 2016.249). Organoids were grown in DMEM/F-12 media with Matrigel (Corning) and seeded in 384 well plates [34 (link)]. After 72 hours of establishment, the organoids were treated with equimolar concentrations of BZA, 5-FU, OX and SN-38 (50 μM - 0 μM) for 7 days. Cell viability was determined using CellTitre-Glo 3D (Promega) according to the manufacturer’s instructions using the EnVision Plate Reader (PerkinElmer). Fluorescence readouts were converted into relative activity using DMSO as a negative control (vehicle treatment) and 1 μM bortezomib as a positive killing control.

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Dmello R.S., Palmieri M., Thilakasiri P.S., Doughty L., Nero T.L., Poh A.R., To S.Q., Lee E.F., Douglas Fairlie W., Mielke L., Parker M.W., Poon I.K., Batlle E., Ernst M, & Chand A.L. (2024). Combination of bazedoxifene with chemotherapy and SMAC-mimetics for the treatment of colorectal cancer. Cell Death & Disease, 15(4), 255.

Publication 2024

Matrigel CellTitre-Glo 3D EnVision Plate Reader

Corresponding Organization :

Other organizations : Olivia Newton-John Cancer Wellness & Research Centre, La Trobe University, Walter and Eliza Hall Institute of Medical Research, University of Melbourne, Institute for Research in Biomedicine

Top 5 similar protocols

Variable analysis

independent variables

Concentrations of BZA, 5-FU, OX and SN-38 (50 μM - 0 μM)

dependent variables

Cell viability

control variables

Patient-derived organoids from resected tumours of patients with colorectal cancer
DMEM/F-12 media with Matrigel (Corning)
Organoids seeded in 384 well plates
Treatment duration of 7 days

positive control

1 μM bortezomib

negative control

DMSO (vehicle treatment)

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